Adaptive strategies of uropathogenic Escherichia coli CFT073: from growth in lab media to virulence during host cell adhesion / Estrategias adaptativas de Escherichia coli CFT073 uropatógena: desde el crecimiento en medios de laboratorio hasta la virulencia durante la adhesión de la célula huésped
Int. microbiol
; 25(3): 481-494, Ago. 2022. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-216208
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Urinary tract infections (UTIs) are a major concern in public health. The prevalent uropathogenic bacterium in healthcare settings is Escherichia coli. The increasing rate of antibiotic-resistant strains demands studies to understand E. coli pathogenesis to drive the development of new therapeutic approaches. This study compared the gene expression profile of selected target genes in the prototype uropathogenic E. coli (UPEC) strain CFT073 grown in Luria Bertani (LB), artificial urine (AU), and during adhesion to host bladder cells by semi-quantitative real-time PCR (RT-PCR) assays. AU effectively supported the growth of strain CFT073 as well as other E. coli strains with different lifestyles, thereby confirming the appropriateness of this medium for in vitro models. Unexpectedly, gene expression of strain CFT073 in LB and AU was quite similar; conversely, during the adhesion assay, adhesins and porins were upregulated, while key global regulators were downregulated with respect to lab media. Interestingly, fimH and papGII genes were significantly expressed in all tested conditions. Taken together, these results provide for the first time insights of the metabolic and pathogenic profile of strain CFT073 during the essential phase of host cell adhesion.(AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Infecções Urinárias
/
Virulência
/
Perfilação da Expressão Gênica
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Fatores de Virulência
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Escherichia coli Uropatogênica
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Bambino Gesù Childrens Hospital/Italy
/
San Raffaele Open University/Italy
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Sapienza University of Rome/Italy