Infection with a human-derived enteroinvasive Escherichia coli strain altered intestinal barrier function in guinea pigs / La infección con una cepa enteroinvasiva de Escherichia coli de origen humano alteró la función de la barrera intestinal en cobayos
Int. microbiol
; 25(4): 723-732, Nov. 2022. graf
Artigo
em Inglês
| IBECS
| ID: ibc-216240
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Background/aims:
The aim was to characterize a bacterium causing intestinal mucosal barrier damage and to identify the possible invasion mechanism. Materials andmethods:
The intestinal permeability and tight junction protein levels were detected in guinea pigs infected with Escherichia coli D-09 via immunofluorescence analysis and western blotting. In order to explain this invasion mechanism at the gene level, whole genome sequencing analysis was performed on this bacterium.Results:
The results showed an increased intestinal permeability and upregulated expression of the leaky protein claudin-2 in both the colon and liver of the infected animals. In addition, the draft genome of E. coli D-09 comprised 42 scaffolds (size, > 645 bp) with a total size of 4,679,567 bp. A total of 4379 protein coding genes were identified, which contained 45 antibiotic resistance and 86 virulence-related genes and covered 88.0% of the whole genome.Conclusions:
This study verified that the human-derived enteroinvasive E. coli strain could destroy intestinal barrier function in guinea pigs. Additionally, our data first characterized the genome features of E. coli O124K72 D-09, which may provide new insights into the possible invasion mechanism.(AU)
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Espanha
Base de dados:
IBECS
Assunto principal:
Colecistite Aguda
/
Escherichia coli
/
Sequenciamento Completo do Genoma
/
Cobaias
/
Mucosa Intestinal
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Hospital of Dalian Medical University/China
/
University of Karachi/Pakistan