Your browser doesn't support javascript.
loading
DC vaccine enhances CAR-T cell antitumor activity by overcoming T cell exhaustion and promoting T cell infiltration in solid tumors
Zhang, Miaomiao; Wang, Yuanyuan; Chen, Xinzu; Zhang, Fan; Chen, Jiannan; Zhu, Hongqiao; Dong, Yunfei; Yin, Xuechen; Ji, Feng; Liu, Jie.
Afiliação
  • Zhang, Miaomiao; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Wang, Yuanyuan; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Chen, Xinzu; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Zhang, Fan; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Chen, Jiannan; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Zhu, Hongqiao; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Dong, Yunfei; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Yin, Xuechen; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Ji, Feng; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
  • Liu, Jie; Nanjing Normal University. College of Life Sciences. Jiangsu Key Laboratory for Molecular and Medical Biotechnology. Nanjing. China
Clin. transl. oncol. (Print) ; 25(10): 2972-2982, oct. 2023.
Artigo em Inglês | IBECS | ID: ibc-225078
Biblioteca responsável: ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Objective Great success has been achieved in CAR-T cell immunotherapy in the treatment of hematological tumors. However, it is particularly difficult in solid tumors, because CAR-T is difficult to enter interior and exert long-term stable immune effects. Dendritic cells (DCs) can not only present tumor antigens but also promote the infiltration of T cells. Therefore, CAR-T cells with the help of DC vaccines are a reliable approach to treat solid tumors. Methods To test whether DC vaccine could promote CAR-T cell therapy in solid tumors, DC vaccine was co-cultured with MSLN CAR-T cells. The in vitro effects of DC vaccine on CAR-T were assessed by measuring cell proliferation, cell differentiation, and cytokine secretion. Effects of DC vaccine on CAR-T were evaluated using mice with subcutaneous tumors in vivo. The infiltration of CAR-T was analyzed using immunofluorescence. The persistence of CAR-T in mouse blood was analyzed using real-time quantitative PCR. Results The results showed that DC vaccine significantly enhanced the proliferation potential of MSLN CAR-T cells in vitro. DC vaccines not only promoted the infiltration of CAR-T cells, but also significantly improved the persistence of CAR-T in solid tumors in vivo. Conclusion In conclusion, this study has demonstrated that DC vaccine can promote CAR-T therapy in solid tumors, which provides the possibility of widespread clinical application of CAR-T cells in the future (AU)
Assuntos

Buscar no Google
Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Vacinas / Imunoterapia Adotiva / Receptores de Antígenos Quiméricos / Neoplasias Limite: Animais Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2023 Tipo de documento: Artigo Instituição/País de afiliação: Nanjing Normal University/China
Buscar no Google
Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Vacinas / Imunoterapia Adotiva / Receptores de Antígenos Quiméricos / Neoplasias Limite: Animais Idioma: Inglês Revista: Clin. transl. oncol. (Print) Ano de publicação: 2023 Tipo de documento: Artigo Instituição/País de afiliação: Nanjing Normal University/China
...