Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR
Int. microbiol
; 12(1): 1-6, mar. 2009. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-72358
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Direct systems to process plant materials allowed high-throughput testing of Plum pox virus (PPV) by real-time reverse transcription (RT)-PCR without nucleic acids purification. Crude plant extracts were diluted in buffer or spotted on membranes to be used as templates. Alternatively, immobilized PPV targets were amplified from fresh sections of plant tissues printed or squashed onto the same supports, without extract preparation. Spot real-time RT-PCR was validated as a PPV diagnostic method in samples collected during the dormancy period and showed high sensitivity (93.6%), specificity (98.0%), and post-test probability (97.9%) towards sharka disease. In an analysis of 2919 Prunus samples by spot real-time RT-PCR and DASI-ELISA 90.8% of the results coincided, demonstrating high agreement (k = 0.77 +/- 0.01) between the two techniques. These results validate the use of immobilized PPV targets and spot real-time RT-PCR as screening method for largescale analyses (AU)
RESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Doenças das Plantas
/
Vírus Eruptivo da Ameixa
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2009
Tipo de documento:
Artigo
Instituição/País de afiliação:
Valencian Institute for Agricultural Research/Spain