Molecular mechanisms of quinolone resistance in clinical isolates of Aeromonas caviae and Aeromonas veronii bv. sobria
Int. microbiol
; 13(3): 135-141, sept. 2010. tab
Artigo
em Espanhol
| IBECS
| ID: ibc-84637
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Mutations in quinolone targets were studied together with quinolone efflux pump activation and plasmid-mediatedquinolone resistance determinants in nalidixic-acid-resistant isolates of Aeromonas caviae and Aeromonas veronii.Among 135 clinical Aeromonas spp. isolated from stools of patients with gastrointestinal symptoms, 40 nalidixic acid-resistantstrains belonging to A. caviae and A. veronii were selected and their susceptibility to different quinolones (ciprofloxacin,norfloxacin, ofloxacin) further evaluated. Susceptibility to nalidixic acid and ciprofloxacin in the presence/absence of Phe-Arg-β-naphthylamide was also determined. The 16 nalidixic-acid-resistant strains identified as A. caviae were more resistantthan the 24 A. veronii bv. sobria strains to ciprofloxacin, norfloxacin, and ofloxacin. All strains showed a mutation (single ordouble) at position 83 of the QRDR sequence of gyrA, with Ser-83 → Ile as the most frequent substitution. By contrast, nomutations were found at position 87 of gyrA. Double substitutions (GyrA-ParC) were detected in 50% of A. veronii bv. sobriaisolates and in 43.75% of A. caviae strains. Both species showed decreases in the MICs of ciprofloxacin. A qnrS gene wasfound in an A. caviae strain. Thus, in the two species of nalidixic-acid-resistant Aeromonas isolates examined, resistancemediated by efflux pumps contributed only slightly to ciprofloxacin resistance. While two isolates were positive for theaac(6′)-Ib gene, no -cr variants were detected (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Infecções por Bactérias Gram-Negativas
/
Quinolonas
/
Aeromonas
/
Farmacorresistência Bacteriana
/
Antibacterianos
Limite:
Humanos
Idioma:
Espanhol
Revista:
Int. microbiol
Ano de publicação:
2010
Tipo de documento:
Artigo
Instituição/País de afiliação:
Lozano Blesa University Clinical Hospital/Spain