Neutrophils infiltration is early event of an oral mucosalxenotransplantation model

ABSTRACT

Introduction: As important effector cells of the innate immune system, neutrophils are involved in rejection of solidorgan and/or tissue transplants. But their role in rejection of oral mucosa transplantation (OMT) remains unclear. Theaim of this study is to observe the spatial-temporal change of neutrophils during acute rejection of OMT.Methods: In a rat model of oral mucosal xenotransplantation, myeloperoxidase (MPO), an indicator of influx ofneutrophils was detected by technique of ELISA on day 7 and 30 (D7, D30) of posttransplantation.Results: On D7, MPO level (6.183±0.416, ×102 ng/mg) in the OMT group was significantly higher than in trauma(0.681±0.073, ×102 ng/mg) and normal controls (0.262±0.043, ×102 ng/mg) (P<0.001, respectively), and this levelwas found to correlate with the index of submandibular lymph nodes (ILN), an indicator of inflammation of rejection(r=0.909, P<0.05). Moreover, this level was decreased significantly under FK506 treatment (2.103±0.146,×102 ng/mg, P= 0.005). On D30, MPO in the OMT group (1.063±0.096, ×102 ng/mg) was lower significantly thanthat on D7 (P<0.001), although this level was still higher that of normal controls on D30 (0.532±0.112, ×102 ng/mg, P = 0.042).Conclusion: Neutrophils infiltration was an early event of OMT, which may play important roles on acute rejectionof OMT (AU)

RESUMEN

No disponible