Expression of the gene encoding secretor type galactoside 2 á fucosyltransferase(FUT2) and ABH antigens in patients with oral lesions
Med. oral patol. oral cir. bucal (Internet)
; 17(1): 63-68, ene. 2012. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-98919
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Objective:
The aim of this work was to evaluate the expression of FUT2 gene in saliva and his to ABH antigens of patients with oral lesions. StudyDesign:
In total 178 subjects were examined, half of whom suffered from oral pre-cancerous and cancerous lesions, while the other half were the healthy control group We analyzed the FUT 2 polymorphism by ASO-PCR(allele specific oligonucleotid - polymerase chain reaction) with specific primers for G428 allele and the wild type allele of FUT2 gene. To reveal A, B and H antigens in tissue sections of the patients (n= 89) we used a modified specific red cell adherence technique.Results:
We found a high intensity of oral disease in the non-secretor group (OR = 2.43). A total of 58% of the patients with oral pre-cancerous and cancerous lesions was non secretors (se_/_), in contrast with the healthy population (21.5%). A strongly positive reaction was defined as a sheet of indicator erythrocytes adhered to the epithelial cells. In 31 of the 54 samples analyzed the test showed slightly positive results on atypical areas, and there was a complete antigen deletion in areas affected by neoplasia. Nineteen samples showed a total absence of ABH antigens in both histologically normal and pathological areas. Blood group antigens were expressed at a high level in benign and highly differentiated malignant tumors. In poorly differentiated malignant tumors, they were mostly absent.Conclusion:
Considering these results we suggest the use of this method to monitor probable preneoplastic lesions in risk population, especially in those with no secretor status (absence of FUT2 gene) (AU)
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Lesões Pré-Cancerosas
/
Neoplasias Bucais
/
Fucosil Galactose alfa-N-Acetilgalactosaminiltransferase
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Inglês
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2012
Tipo de documento:
Artigo
Instituição/País de afiliação:
National University of Rosario/Argentina