Evaluation of different immunization protocols with P. brasiliensis antigens in guinea pigs
Rev. Inst. Med. Trop. Säo Paulo
; 34(3): 243-9, maio-jun. 1992. tab
Artigo
em Inglês
| LILACS
| ID: lil-134512
Biblioteca responsável:
BR66.1
RESUMO
The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable length of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immunodiffusion, skin test and macrophage migration inhibition test. It was observed that 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Paracoccidioidomicose
Base de dados:
LILACS
Assunto principal:
Paracoccidioides
/
Imunização
/
Antígenos de Fungos
Tipo de estudo:
Estudo de etiologia
/
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
1992
Tipo de documento:
Artigo