Diameter of the mammalian porin channel in open and "closed" states: direct measurement at the single channel level in planar lipid bilayer
Braz. j. med. biol. res
; 29(12): 1691-7, Dec. 1996. graf
Article
em En
| LILACS
| ID: lil-188456
Biblioteca responsável:
BR1.1
RESUMO
Porin isolated from bovine skeletal muscle was reconstituted in planar lipid bilayers under voltage clamp conditions. A set of non-electrolytes were used as molecular probes for determining the pore diameter. The maximal diameter of the open channel was estimated to be 3.02 + 0.26 nm. As observed for other porin channels, a large transmembrane potential drove the channel into a "closed" state. The channel transition to the low conductance (closed) state was followed by a decrease in the maximal diameter of the channel to 2.4 +- 0.08 nm.
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
/
Porinas
/
Canais Iônicos
/
Bicamadas Lipídicas
/
Mamíferos
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
Brasil