Actions of Crotalus durissus terrificus venom and crotoxin on the isolated rat kidney
Braz. j. med. biol. res
; 34(10): 1347-1352, Oct. 2001. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-299838
Biblioteca responsável:
BR1.1
RESUMO
Many studies have reported the occurrence of lethal acute renal failure after snakebites. The aim of the present investigation was to determine alterations in renal function produced by Crotalus durissus terrificus venom and crotoxin as well as the histological alterations induced by these venoms. Isolated kidneys from Wistar rats weighing 240 to 280 g were perfused with Krebs-Henseleit solution containing 6 g percent of previously dialyzed bovine serum albumin. The effects of Crotalus durissus terrificus venom and crotoxin were studied on glomerular filtration rate (GFR), urinary flow (UF), perfusion pressure (PP) and percentage sodium tubular transport ( percentTNa+). The infusion of Crotalus durissus terrificus venom (10 æg/ml) and crotoxin (10 æg/ml) increased GFR (control80 = 0.78 + or - 0.07, venom80 = 1.1 + or - 0.07, crotoxin80 = 2.0 + or - 0.05 ml g-1 min-1, P<0.05) and UF (control80 = 0.20 + or - 0.02, venom80 = 0.32 + or - 0.03, crotoxin80 = 0.70 + or - 0.05 ml g-1 min-1, P<0.05), and decreased percentTNa+ (control100 = 75.0 + or - 2.3, venom100 = 62.9 + or - 1.0, crotoxin80 = 69.0 + or - 1.0 ml g-1 min-1, P<0.05). The infusion of crude venom tended to reduce PP, although the effect was not significant, whereas with crotoxin PP remained stable during the 100 min of perfusion. The kidneys perfused with crude venom and crotoxin showed abundant protein material in the urinary space and tubules. We conclude that Crotalus durissus terrificus venom and crotoxin, its major component, cause acute nephrotoxicity in the isolated rat kidney. The current experiments demonstrate a direct effect of venom and crotoxin on the perfused isolated kidney
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Crotalus
/
Venenos de Crotalídeos
/
Rim
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2001
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual do Ceará/BR
/
Universidade Federal do Ceará/BR
/
Universidade de Fortaleza/BR