First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women
Mem. Inst. Oswaldo Cruz
; 97(7): 985-989, Oct. 2002. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-325908
Biblioteca responsável:
BR1.1
RESUMO
A total of 106 women with vaginitis in Nicaragua were studied. The positive rate for the identification of Candida species was 41 percent (44 positive cultures out of 106 women with vaginitis). The sensitivity of microscopic examination of wet mount with the potassium hydroxide (KOH) was 61 percent and 70 percent with Gram's stain when using the culture of vaginal fluid as gold standard for diagnosis of candidiasis. Among the 44 positives cultures, isolated species of yeast from vaginal swabs were C. albicans (59 percent), C. tropicalis (23 percent), C. glabrata (14 percent) and C. krusei (4 percent). This study reports the first characterization of 26 C. albicans stocks from Nicaragua by the random amplified polymorphic DNA method. The genetic analysis in this small C. albicans population showed the existence of linkage disequilibrium, which is consistent with the hypothesis that C. albicans undergoes a clonal propagation
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Candida albicans
/
Candidíase Vulvovaginal
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América Central
/
Nicarágua
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
França
/
Nicarágua
Instituição/País de afiliação:
Institut de Recherche pour le Développement/FR
/
Laboratoire de Parasitologie Médicale et d'Ecologie Humaine/FR
/
Ministerio de Salud de Nicaragua/NI