Microsporogénesis y microgametogénesis de onoto (Bixa orellana L. ) / Microsporogenesis and microgametogenesis of annatto (Bixa orellana L. )
Acta cient. venez
; 53(3): 171-175, 2002. ilus
Artigo
em Espanhol
| LILACS
| ID: lil-331334
Biblioteca responsável:
BR1.1
ABSTRACT
A series of buds of increasing maturity were individually sampled in order to examine cytological events of annatto (Bixa orellana L.), genotype Portuguesa. They were fixed in Carnoy II at 1230 am, time of the highest rate of meiotic division. Three stain solutions were attempted. In the microspores mother cells, the use of acetic orcein 1% resulted in a good nucleus coloration and sharpness. In contrast, a well chromosome resolution was achieved with the application of propionic carmin 2%. The pollen grain mother cells (n = 8 chromosomes) at metaphase I were found in floral buds of 0.5 to 0.6 cm long; tetrad stage in buds of 0.6 to 0.7 cm long, uninucleate stage of microspores in buds of 0.7 to 0.8 cm long and the binucleate stage (pollen) in buds longer than 0.8 cm. Microphotographies showing the sequence of meiotic division (microsporogenesis) and subsequent mitosis to originate pollen grains were included
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Pólen
/
Divisão Celular
/
Cromossomos
/
Bixaceae
Idioma:
Espanhol
Revista:
Acta cient. venez
Assunto da revista:
Ciência
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Venezuela
Instituição/País de afiliação:
Universidad Central de Venezuela/VE