Expression and activity of matrix metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
Braz. dent. j
; 13(2): 97-102, 2002.
Artigo
em Inglês
| LILACS
| ID: lil-332153
Biblioteca responsável:
BR97.1
RESUMO
Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Germe de Dente
/
Metaloproteinase 2 da Matriz
/
Dente Molar
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. dent. j
Assunto da revista:
Odontologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
State University of Campinas-UNICAMP/BR