Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment
Biocell
; 26(2): 217-223, Aug. 2002.
Artigo
em Inglês
| LILACS
| ID: lil-335851
Biblioteca responsável:
BR1.1
ABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Triticum
/
Plantas Geneticamente Modificadas
/
Aminobutiratos
/
Herbicidas
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Instituto de Fitopatología y Fisiología Vegetal/AR