Microwave-enhanced of a method of silver impregnation applied in dermatohistopathology
Biocell
; 23(2): 143-147, Aug. 1999.
Artigo
em Inglês
| LILACS
| ID: lil-340370
Biblioteca responsável:
AR5.1
RESUMO
An experimental development based on the combination of microwaves action with one of the methods of silver staining by Del RÝo Hortega is presented. Materials from normal and pathological tissues were studied. The stuff were fixed in 10 formalin and received a paraffined embedding. The impregnation was made with ammoniacal silver carbonate in a domestic microwave oven during twelve seconds at 375 w. As a result of this, a time shortage of the technique, the high degree of definition of the nuclear, cytoplasmatic and extracellular matrix (collagenus fibbers with an unsteady reticular representation) details and the easy visualization of mitosis was observed. Also, omitting the reduction, they were clearly evidenced the argirophilics granules (melanin pigment)
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Neoplasias Cutâneas
/
Carcinoma Basocelular
/
Carcinoma de Células Escamosas
/
Fixação de Tecidos
/
Coloração pela Prata
/
Micro-Ondas
Limite:
Humanos
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Laboratorio de Diagnóstico e Investigaciones Histopatológicas/AR