Age-related biological activity of south american rattlesnake (Crotalus durissus terrificus) venom
J. venom. anim. toxins incl. trop. dis
; 9(2): 186-201, 2003. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-345737
Biblioteca responsável:
BR33.1
ABSTRACT
An in vitro and in vivo comparative study was performed on the effects of Crotalus durissus terrificus venoms from a mother and its 15 newborns. The venoms were tested for protein content, lethality, proteolytic, myotoxic, hemorrhagic, and phospholipase A2 activity. The minimum coagulant dose in plasma and human fibrinogen, protrhombin, and Factor II activations were analyzed. The venoms were also analyzed by polyacrylamide gel electrophoresis (PAGE). This showed that despite similar total protein content, the biological effects of the venoms were different. Venom from young snakes exhibited higher enzymatic and coagulant activities and higher myotoxicity compared to the mother's. In addition, the PLA2 content paralleled myotoxicity. However, no difference could be detected in their toxicity (LD50 0.08 mg/Kg). High incidence of blood coagulation disorders and elevated circulating myoglobin may characterize systemic envenoming by young C. d. terrificus.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Coagulação Sanguínea
/
Crotalus
/
Venenos de Crotalídeos
Limite:
Animais
País/Região como assunto:
América do Sul
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Reino Unido
Instituição/País de afiliação:
Intituto Butantan/BR
/
Universidade do Amazonas/BR
/
University of Liverpool/GB