Cord formation and colony morphology for the presumptive identification of Mycobacterium tuberculosis complex
Braz. j. microbiol
; 34(2): 171-174, Apr.-Jun. 2003. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-355168
Biblioteca responsável:
BR32.1
RESUMEN
The identification of Mycobacterium tuberculosis complex (MT), using non-molecular methods, is time-consuming. The objective of this study was to evaluate a screening test for the presumptive identification of MT, which could potentially decrease laboratory turn-around time for reporting preliminary results. From January 1998 to December 1999, 3056 cultures were analysed at the Mycobacterial Laboratory, Instituto Adolfo Lutz, S los o Paulo, Brasil. The screening test consisted of observation of colony morphology on L que wenstein Jensen medium and evaluation of cord formation on smear microscopy from those positive cultures. After the screening test, the cultures identified as non-tuberculous mycobacteria were identified to species by conventional methods (growth on culture and biochemical tests). Those identified as MT were submitted to drug susceptibility tests. The presumptive identification of MT using the proposed screening test, when compared with conventional tests, presented 98.9, 86.9, 97.8 and 93.0 percent of sensitivity, specificity, positive and negative predictive values, respectively. The conclusion is that it is possible to make a presumptive identification of MT using visual analysis of colony morphology and cord formation on microscopy examination. This method could be used to report the presumptive identification of MT and to guide laboratory decisions regarding susceptibility and identification tests with little cost and in a very practical way.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Doenças Negligenciadas
/
Tuberculose
Base de dados:
LILACS
Assunto principal:
Técnicas Bacteriológicas
/
Fatores Corda
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Instituto Adolfo Lutz/BR