Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans
Braz. j. med. biol. res
; 37(10): 1455-1461, Oct. 2004. ilus
Artigo
em Inglês
| LILACS
| ID: lil-383034
Biblioteca responsável:
BR1.1
ABSTRACT
The respiration, membrane potential (FACE="Symbol">Dy), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05 percent BSA. Mitochondria in situ generated and sustained stable mitochondrial FACE="Symbol">Dy respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 µM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 µM) inhibited respiration by 30 percent and 2 µM antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85 percent. Cyanide-insensitive respiration was partially blocked by 2 mM benzohydroxamic acid, suggesting the presence of an alternative oxidase. Candida albicans mitochondria in situ presented a carboxyatractyloside-insensitive increase of FACE="Symbol">Dy induced by 5 mM ATP and 0.5 percent BSA, and FACE="Symbol">Dy decrease induced by 10 µM linoleic acid, both suggesting the existence of an uncoupling protein. The presence of this protein was subsequently confirmed by immunodetection and respiration experiments with isolated mitochondria. In conclusion, Candida albicans ATCC 90028 possesses an alternative electron transfer chain and alternative oxidase, both absent in animal cells. These pathways can be exceptional targets for the design of new chemotherapeutic agents. Blockage of these respiratory pathways together with inhibition of the uncoupling protein (another potential target for drug design) could lead to increased production of reactive oxygen species, dysfunction of Candida mitochondria, and possibly to oxidative cell death.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Fosforilação Oxidativa
/
Esferoplastos
/
Candida albicans
/
Espécies Reativas de Oxigênio
/
Potenciais da Membrana
/
Mitocôndrias
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR