Long-term ethanol intoxication reduces inflammatory responses in rats
Braz. j. med. biol. res
; 38(1): 81-89, Jan. 2005. graf
Artigo
em Inglês
| LILACS
| ID: lil-405535
Biblioteca responsável:
BR1.1
ABSTRACT
The anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing concentrations of ethanol vapor over a 10-day period. One group was evaluated immediately after exposure (treated group - intoxicated), and another was studied 7 h later (withdrawal group). Ethanol inhalation treatment significantly inhibited carrageenan- (62 percent for the intoxicated group, N = 5, and 35 percent for the withdrawal group, N = 6) and dextran-induced paw edema (32 percent for intoxicated rats and 26 percent for withdrawal rats, N = 5 per group). Ethanol inhalation significantly reduced carrageenan-induced neutrophil migration (95 percent for intoxicated rats and 41 percent for withdrawn rats, N = 6 per group) into a subcutaneous 6-day-old air pouch, and Sephadex-induced eosinophil migration to the rat peritoneal cavity (100 percent for intoxicated rats and 64 percent for withdrawn rats, N = 6 per group). A significant decrease of mast cell degranulation was also demonstrated (control, 82 percent; intoxicated, 49 percent; withdrawn, 51 percent, N = 6, 6 and 8, respectively). Total leukocyte and neutrophil counts in venous blood increased significantly during the 10 days of ethanol inhalation (leukocytes, 13, 27 and 40 percent; neutrophils, 42, 238 and 252 percent, respectively, on days 5, 9 and 10, N = 7, 6 and 6). The cell counts decreased during withdrawal, but were still significantly elevated (leukocytes, 10 percent; neutrophils, 246 percent, N = 6). These findings indicate that both the cellular and vascular components of the inflammatory response are compromised by long-term ethanol intoxication and remain reduced during the withdrawal period.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Degranulação Celular
/
Etanol
/
Intoxicação Alcoólica
/
Edema
/
Inflamação
/
Mastócitos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2005
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Ceará/BR