Trypanosoma cruzi strains isolated from human, vector, and animal reservoir in the same endemic region in Mexico and typed as T. cruzi I, discrete typing unit 1 exhibit considerable biological diversity
Mem. Inst. Oswaldo Cruz
; 101(6): 585-590, Sept. 2006. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-437049
Biblioteca responsável:
BR1.1
ABSTRACT
In this study, three strains of Trypanosoma cruzi were isolated at the same time and in the same endemic region in Mexico from a human patient with chronic chagasic cardiomyopathy (RyC-H); vector (Triatoma barberi) (RyC-V); and rodent reservoir (Peromyscus peromyscus) (RyC-R). The three strains were characterized by multilocus enzyme electrophoresis, random amplified polymorphic DNA, and by pathological profiles in experimental animals (biodemes). Based on the analysis of genetic markers the three parasite strains were typed as belonging to T. cruzi I major group, discrete typing unit 1. The pathological profile of RyC-H and RyC-V strains indicated medium virulence and low mortality and, accordingly, the strains should be considered as belonging to biodeme Type III. On the other hand, the parasites from RyC-R strain induced more severe inflammatory processes and high mortality (> 40 percent) and were considered as belonging to biodeme Type II. The relationship between genotypes and biological characteristics in T. cruzi strains is still debated and not clearly understood. An expert committee recommended in 1999 that Biodeme Type III would correspond to T. cruzi I group, whereas Biodeme Type II, to T. cruzi II group. Our findings suggest that, at least for Mexican isolates, this correlation does not stand and that biological characteristics such as pathogenicity and virulence could be determined by factors different from those identified in the genotypic characterization
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doença de Chagas
/
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Trypanosoma cruzi
/
Variação Genética
/
Doença de Chagas
/
Parasitemia
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
País/Região como assunto:
México
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2006
Tipo de documento:
Artigo
País de afiliação:
França
/
México
Instituição/País de afiliação:
Benemérita Universidad Autónoma de Puebla/MX
/
Centre d'Etudes sur le Polymorphisme des Micro-Organismes/FR
/
Centro de Investigaciones Regionales/MX
/
Instituto Mexicano del Seguro Social/MX