Recombinant expression of marine shrimp lysozyme in Escherichia coli
Electron. j. biotechnol
; 7(3): 12-13, Dec. 2004. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-448769
Biblioteca responsável:
CL1.1
ABSTRACT
Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10 percent. Details of the protein recombinant expression techniques applied to this shrimp protein are presented.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Proteínas Recombinantes
/
Muramidase
/
Penaeidae
/
Escherichia coli
Limite:
Animais
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
Centro de Investigación en Alimentación y Desarrollo A.C/MX
/
Centro de Investigación en Alimentación y Desarrollo, A.C/MX
/
Universidad de Sonora/MX