rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro
J. venom. anim. toxins incl. trop. dis
; 14(1): 113-127, 2008. graf, tab
Article
em En
| LILACS
| ID: lil-479343
Biblioteca responsável:
BR33.1
ABSTRACT
Snake venom metalloproteases (SVMPs) comprise a family of snake venom toxins responsible for most of local and systemic effects observed during envenomation by snakes from the Viperidae family. The vascular system and more specifically the endothelium seem to be the preferential targets of these proteins. This work describes the effects of rACLF, a recombinant SVMP from Agkistrodon contortrix laticinctus on human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that rACLF activates HUVECs by the release of mediators involved in inflammation and hemostasis such as prostacyclin and interleukin-8. We also demonstrated that rACLF increased the expression of ICAM-I and decay accelerating factor (DAF). Moreover, rACLF protects the HUVECs against apoptosis induced by serum deprivation. These results suggest that the endothelial cell activation induced by SVMPs may have a significant role in the development of the local inflammatory lesion observed in Viperidae envenomation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Proteínas Recombinantes
/
Venenos de Crotalídeos
/
Células Endoteliais
/
Metaloproteases
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
/
Project document
País de afiliação:
Brasil
País de publicação:
Brasil