Oncogene-induced transcriptional patterns in established cell lines as a model for in vitro analysis of tumor biology
Genet. mol. res. (Online)
; 3(3): 410-420, 2004. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-482168
Biblioteca responsável:
BR1.1
ABSTRACT
Established cell lines have long been used for in vitro studies of tumor biology, enabling investigators to control growth conditions and to draw important conclusions about the oncogenic microenvironment. However, gene expression behavior in cultured cells may not always reflect the actual in vivo scenario, and analysis derived from such experiments should take into consideration the existing differences between the two environments. We used suppression subtractive hybridization to study transcriptional changes elicited after oncogene transformation and cell line establishment. We found that transcriptional changes elicited in cultured cell lines are in fact representative of late events, and they do not occur early after oncogene transfection or activation. We also determined that a fraction of the transcriptional changes is oncogene specific, whereas other changes are shared between two or more different oncogenes.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Oncogenes
/
Transcrição Gênica
/
Transformação Celular Neoplásica
Limite:
Humanos
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2004
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UNIFERT/BR
/
Universidade Federal do Espírito Santo/BR