Heparin and commercial bothropic antivenom against the paralyzing effect of Bothrops jararacussu snake venom
J. venom. anim. toxins incl. trop. dis
; 16(1): 34-45, 2010. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-542435
Biblioteca responsável:
BR33.1
ABSTRACT
The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50 percent neuromuscular blockade induced by Bjssu crude venom received 1 µL/mL of heparin while Group 5 received a mixture of HepCBABjssu. Control preparations (Tyrode) were treated with Hep and CBA (mean ± SEM; n = 3-6). After 120 minutes of venom incubation, Group 1 preparations presented twitch-tension of 12 ± 2 percent. However, in Groups 2 and 3, the neutralizations were 92 ± 1.9 percent and 81 ± 6 percent, respectively. The heparin addition, after 50 percent neuromuscular blockade by Bjssu, produced 40 ± 6 percent muscular response after 120 minutes of incubation. HepCBABjssu mixture displayed a protective effect of 84 ± 10 percent against venom action. In conclusion, heparin and commercial bothropic antivenom efficiently neutralized the neurotoxic effects caused by B. jararacussu crude venom, even at different incubation time-points.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Antivenenos
/
Heparina
/
Bothrops
/
Venenos de Crotalídeos
Tipo de estudo:
Guia de prática clínica
Limite:
Animais
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
State University of Campinas/BR