Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
Clinics
; Clinics;65(3): 305-309, 2010. ilus
Article
em En
| LILACS
| ID: lil-544010
Biblioteca responsável:
BR1.1
ABSTRACT
INTRODUCTION:
Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60 percent of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential.OBJECTIVE:
To develop an experimental malignant astrocytoma model with the characteristics of the human tumor.METHOD:
Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats.RESULTS:
All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed.CONCLUSION:
A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Astrocitoma
/
Neoplasias Encefálicas
/
Hospedeiro Imunocomprometido
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Glioblastoma
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Clinics
Assunto da revista:
MEDICINA
Ano de publicação:
2010
Tipo de documento:
Article
/
Project document
País de afiliação:
Brasil
País de publicação:
Brasil