Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
Mem. Inst. Oswaldo Cruz
; 105(3): 331-335, May 2010. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-547306
Biblioteca responsável:
BR1.1
ABSTRACT
The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87 percent of the antral samples from Cuban patients and 80.3 percent of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Proteínas de Bactérias
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Gastrite
/
Antígenos de Bactérias
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Caribe
/
Cuba
/
Venezuela
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Cuba
/
França
/
México
/
Venezuela
Instituição/País de afiliação:
INSERM/FR
/
Instituto Mexicano del Seguro Social/MX
/
United Nations Framework Convention on Climate Change de La Habana/CU
/
Universidad Central de Venezuela/VE