PCR duplex para diferenciação de amostras vacinais e selvagens do vírus da doença de Aujeszky / A duplex PCR for differentiation of wild and vaccine virus of Aujeszkyïs disease
Arq. bras. med. vet. zootec
; 62(5): 1259-1262, out. 2010. ilus
Artigo
em Português
| LILACS
| ID: lil-570488
Biblioteca responsável:
BR68.1
ABSTRACT
A duplex PCR was developed to differentiate the wild-type virus from the attenuated virus used in vaccinations. The PCR was able to amplify fragments of 493bp for glycoprotein E (gE) gene and 207bp for glycoprotein B (gB) gene. The analytical sensitivity was determined by addition of a virus field sample titled in the brain samples of pigs. The standard virus strain Shope, the vaccine strain Bartha, and ten other field isolates were subjected to PCR. The PCR was able to amplify fragments of gE and gB in all field samples and only fragments of gB were amplified in the attenuated virus, as expected. The technique was able to detect up to 100.5 TCID50/50mL virus in samples of brain. Duplex PCR proved to be an important tool for differentiation of naturally-infected animals and animals vaccinated with the virus deleted for gE.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
Base de dados:
LILACS
Assunto principal:
Reação em Cadeia da Polimerase
/
Herpesvirus Suídeo 1
Limite:
Animais
Idioma:
Português
Revista:
Arq. bras. med. vet. zootec
Assunto da revista:
Medicina Veterinária
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Ministerio da Agricultura, Pecuaria e Abastecimento/BR
/
Universidade Federal de Minas Gerais/BR