Algae acid phosphatase affected by ten metals and enzymatic parameters determination in presence of mercury and copper
Pesticidas
; 19: 1-10, jan.-dez. 2009. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-575996
Biblioteca responsável:
BR16.1
ABSTRACT
Acid phosphatases play important roles in algae metabolism such availability/recycling of inorganic phosphate and autophagic digestive processes. Chemicals released into the environment from agricultural activities and through industrial and urban wastes, may impair algae enzyme activity. The aim of this work was to evaluate the in vitro activation/inhibition effect of ten metals, commonly present as contaminants in soil and water, on the acid phospahatase extracted from the green algae Pseudokirchneriella subcapitata. Results demonstrated that Hg, Al, Mo, Pb, Se, and Cd inhibited the enzyme activity in 56.3, 54.5, 30.6, 25.5, 23.1 and 11.5 per cent respectively. This corresponds to the maximum percentage of effect attained at the metal concentration tested (0.02-2.0 mM). On the other hand, Cu, Zn, Ni and Cr exhibited an increment on phosphatase activity equal to 95.5, 87.6, 87.6, 77.6 and 42.8 per cent, respectively. Kinetic parameters values were calculated for the metals that showed hisghest effects. Thus, Ki ( inhibition constant) and Kd (dissociation constant) values equal to 0.0400 and 0.0016 mM were determined for Hg and Cu, respectively. A non-competitive inhibition mechanism was attributed to the former. Results improved the understanding of the basic events of the impact of metals at biochemical lebels in primary producers organisms.
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Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.9: Reduzir o número de mortes por produtos químicos perigosos e contaminação do ar e água do solo
Base de dados:
LILACS
Assunto principal:
Fitoplâncton
/
Poluentes Ambientais
/
Enzimas
Idioma:
Inglês
Revista:
Pesticidas
Assunto da revista:
Meio Ambiente
/
Praguicidas
/
Toxicologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Empresa Brasileira de Pesquisa Agropecuária/BR
/
Universidade Estadual de Campinas/BR