Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4)
J. venom. anim. toxins incl. trop. dis
; 17(3): 300-307, 2011. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-597229
Biblioteca responsável:
BR33.1
ABSTRACT
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls, L. major seemed more sensitive to the toxic effects of AlPcS4 in the dark. The most effective photodynamic therapy (PDT) was obtained with AlPcS4 at 10.0 µM and 10 J/cm² whereas L. braziliensis showed the highest mortality rate after treatment.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Leishmaniose
/
Doenças da Pele
Base de dados:
LILACS
Assunto principal:
Fotoquimioterapia
/
Leishmania braziliensis
/
Leishmaniose Cutânea
/
Fármacos Fotossensibilizantes
/
Leishmania major
/
Antiparasitários
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Vale do Paraíba University/BR