Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues
Mem. Inst. Oswaldo Cruz
; 107(1): 85-88, Feb. 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-612810
Biblioteca responsável:
BR1.1
ABSTRACT
In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfully detected Leptospira in four dubious cases of human leptospirosis from archival tissue specimens and one leptospirosis-positive canine specimen. A sensitive method for leptospirosis identification in FFPE tissues would be a useful tool to screen histological specimen archives and gain a better assessment of human leptospirosis prevalence, especially in tropical countries, where large outbreaks can occur following the rainy season.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Reação em Cadeia da Polimerase
/
Rim
/
Leptospira interrogans
/
Pulmão
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Cuba
/
Itália
Instituição/País de afiliação:
Institute of Tropical Medicine Pedro Kourì/CU
/
Istituto Zooprofilattico Sperimentale della Sicilia A.Mirri/IT
/
Università di Palermo/IT