Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes
Rev. Inst. Adolfo Lutz
; 71(2): 250-258, abr.-jun. 2012. tab
Article
em En
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-688220
Biblioteca responsável:
BR76.1
ABSTRACT
This work aimed at isolating Staphylococcus spp. from minas frescal type cheese, and for this purpose a Multiplex PCR (M-PCR) was standardized for detecting the classical Staphylococcus aureus enterotoxingenes (sea, seb, sec, sed and see), using the femA gene as a positive control for S. aureus strains. Bacterial detection directly from the minas frescal type cheese and from artificially contaminated food was tested.One hundred eleven colonies (104 coagulase-positive and seven coagulase-negative) were selected for performing M-PCR assay. Thirty-four colonies (30.62%) were positive for at least one of the fiveente rotoxin genes analyzed; enterotoxins sea and seb were the most frequently detected. The study on Staphylococci coagulase-positive samples revealed that 40% of the samples showed bacterial counts above the limit established by Brazilian legislation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
/
SES-SP
/
SESSP-ACVSES
/
SESSP-CTDPROD
/
SESSP-IALACERVO
/
SESSP-IALPROD
Assunto principal:
Staphylococcus
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Queijo
/
Reação em Cadeia da Polimerase
/
Enterotoxinas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Rev. Inst. Adolfo Lutz
Assunto da revista:
MICROBIOLOGIA
/
SAUDE PUBLICA
Ano de publicação:
2012
Tipo de documento:
Article
País de publicação:
Brasil