Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process
Acta cir. bras
; Acta cir. bras;29(supl.1): 29-33, 2014. graf
Article
em En
| LILACS
| ID: lil-720398
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To evaluate the effects of hyperbaric oxygen on rats submitted to hepatic ischemia and reperfusion.METHODS:
Twenty-three Wistar rats were divided at random into 3 groups SHAM, rats submitted to surgical and anesthetic stress without induction of hepatic ischemia/reperfurion; I/R, rats submitted to total ischemia of the hepatic pedicle for 25 min followed by 5 min of reperfusion; HBOI/R, rats submitted to 60 min of hyperbaric oxygen therapy at a pressure of 2 absolute atmospheres immediately after the experimental protocol of ischemia/reperfusion. Hepatic function was evaluated by quantitation of serum alanine aminotranferase (ALT) and aspartate aminotransferase (AST), and by mitochondrial function through the determination of states 3 and 4 of mitochondrial respiration, respiratory control ratio (RCR) and mitochondrial swelling. Data were analyzed by the Mann-Whitney test, with the level of significance set at p <0.05.RESULTS:
There was a significant difference in state 3 values for the SHAM group vs I/R and I/R vs IRHBO, in state 4 values for the SHAM group vs I/R; and in mitochondrial swelling for the SHAM groups vs I/RHBO, SHAM vs I/R, and IR vs I/RHBO.CONCLUSION:
The use of hyperbaric oxygen after I/R improved in a relative manner both the production of energy and the effects on the mitochondrial wall. .Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Traumatismo por Reperfusão
/
Oxigenoterapia Hiperbárica
/
Fígado
Tipo de estudo:
Evaluation_studies
/
Guideline
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil