Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?
Acta cir. bras
; 30(4): 247-252, 04/2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-744281
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.METHODS:
Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.RESULTS:
MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL- β) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.CONCLUSION:
Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects. .
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Metotrexato
/
Ácido Tióctico
/
Doença Hepática Induzida por Substâncias e Drogas
/
Antimetabólitos Antineoplásicos
/
Antioxidantes
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Antalya Training and Research Hospital/TR