ELISA detection of specific circulating antibodies against Schistosoma mansoni in mice after treatment with oxamniquine
Braz. j. med. biol. res
; 24(5): 485-93, 1991. ilus
Artigo
em Inglês
| LILACS
| ID: lil-99480
Biblioteca responsável:
BR26.1
RESUMO
Mice infected with 80 cercariae of Schistosoma mansoni treated with a single oral dose of oxamnique (400 mg/kg) 65 days after infection. Groups of 8-12 animals were sacrificed approximately 2 weeks after treatment and then at montly intervals. The sera obtained were evaluated for S. mansoni antibodies by enzyme-linked immunosorbeent assay (ELISA) at 1200 dilution. Worms could not be recovered on days 14, 28, 58, 90, 119, 154 and 180 after treatment, indicating the efficacy of the chemotherapy. When performed with different antigens obtained from several stages in the life cycle of S. mansoni, i.e., soluble egg antigen, adult worm tegument, cercaria antigen, schistosomule tegument and adult worm (10 *g antigen/ml), the ELISA showed a decrease in specific antibody levels as a function of time after treatment starting on day 58, reaching levels close to control (noninfected untreated) in most animals 120 days after treatment. Purified antigens from the adult worm and the schistosomule tegument appear to be promising for use in clinical studies evaluating schistosomiasis after drug treatment
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Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Oxamniquine
/
Schistosoma mansoni
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1991
Tipo de documento:
Artigo