Chromatographic purification and characterization of B-phycoerythrin from Porphyridium cruentum. Semipreparative high-performance liquid chromatographic separation and characterization of its subunits.
J Chromatogr A
; 917(1-2): 135-45, 2001 May 11.
Article
em En
| MEDLINE
| ID: mdl-11403465
A fast preparative two-step chromatographic method for purification of B-phycoerythrin from Porphyridium cruentum is described. This biliprotein was homogeneous as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielding three closely migrating bands corresponding to its three subunits. Baseline separation of its alpha-, beta- and gamma-subunits was achieved by a reversed-phase HPLC gradient semipreparative method with a C4 large-pore column and a solvent system consisting of 0.05% trifluoroacetic acid (TFA) in water and 0.05% TFA in acetonitrile. B-Phycoerythrin in different aggregation states and its subunits have been spectroscopically characterized. Hexameric B-phycoerythrin has similar secondary and tertiary structure than dissociated B-phycoerythrin determined by circular dichroism.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ficoeritrina
/
Cromatografia Líquida de Alta Pressão
/
Rodófitas
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Espanha
País de publicação:
Holanda