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Extraintestinal rotavirus infections in children with immunodeficiency.
Gilger, M A; Matson, D O; Conner, M E; Rosenblatt, H M; Finegold, M J; Estes, M K.
Afiliação
  • Gilger MA; Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
J Pediatr ; 120(6): 912-7, 1992 Jun.
Article em En | MEDLINE | ID: mdl-1317419
Some rotavirus strains, including vaccine candidates, have been demonstrated to cause hepatitis in immunodeficient and malnourished mice and to grow in human liver cells. To determine whether rotavirus spreads outside the intestine in naturally infected children, we examined tissues from four immunodeficient children affected with severe combined immunodeficiency disease, acquired immunodeficiency disease syndrome, or DiGeorge syndrome. Chronic rotavirus-related diarrhea, which persisted until death, had also developed in each child. Using indirect immunoperoxidase techniques, we identified rotavirus antigen in the liver and kidney with a hyperimmune guinea pig antiserum prepared to double-shelled rotavirus particles. Similar immunostaining with an antiserum to a rotavirus nonstructural protein (NS26) provided evidence of active virus replication. The observed reactivity was eliminated specifically when serial sections were immunostained with the same antiserum that had been absorbed with either double-shelled rotavirus particles or NS26. Immunostaining was not observed in the liver of children with other diseases (alpha 1-antitrypsin deficiency, inspissated bile syndrome, and acute rejection of a transplanted liver). These findings demonstrate that rotavirus infections in children can extend beyond the intestinal tract. Further studies are warranted to determine whether extraintestinal rotavirus replication occurs in children without severe immunodeficiency, such as malnourished children.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Síndrome da Imunodeficiência Adquirida / Imunodeficiência Combinada Severa / Síndrome de DiGeorge / Hepatite Viral Humana / Nefropatias Tipo de estudo: Prognostic_studies Limite: Female / Humans / Infant / Male Idioma: En Revista: J Pediatr Ano de publicação: 1992 Tipo de documento: Article País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Síndrome da Imunodeficiência Adquirida / Imunodeficiência Combinada Severa / Síndrome de DiGeorge / Hepatite Viral Humana / Nefropatias Tipo de estudo: Prognostic_studies Limite: Female / Humans / Infant / Male Idioma: En Revista: J Pediatr Ano de publicação: 1992 Tipo de documento: Article País de publicação: Estados Unidos