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OKT3-induced cytokine mRNA expression in human peripheral blood mononuclear cells measured by polymerase chain reaction.
Pisa, E K; Pisa, P; Hansson, M; Wigzell, H.
Afiliação
  • Pisa EK; Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Scand J Immunol ; 36(5): 745-9, 1992 Nov.
Article em En | MEDLINE | ID: mdl-1439586
The kinetic profile of cytokine gene expression in normal human peripheral mononuclear cells (MNC) activated by an anti-CD3 monoclonal antibody was studied. The presence or absence of 10 different cytokine mRNA were measured in a polymerase chain reaction (PCR) assisted mRNA amplification assay. After 2 h of stimulation the mRNA for interleukin-1 alpha (IL-1 alpha), interleukin-2 (IL-2), interleukin-3 (IL-3), tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) were detectable and remained present during the whole time period studied (22 h). Interleukin-6 (IL-6) and granulocyte macrophage colony stimulating factor (GM-CSF) were detected after 4 h, while interleukin-10 (IL-10) mRNA did not appear until after 7 h; they all remained expressed at 22 h. A transient expression of interleukin-4 (IL-4) mRNA was observed between 4 and 7 h of stimulation. No gene expression of granulocyte colony stimulating factor (G-CSF) was detected at any time. These results show that anti-CD3 stimulation of MNC leads to a rapid sequential induction of different cytokine mRNA, some with a very transient expression.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Leucócitos Mononucleares / Citocinas / Muromonab-CD3 Limite: Humans Idioma: En Revista: Scand J Immunol Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Suécia País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Leucócitos Mononucleares / Citocinas / Muromonab-CD3 Limite: Humans Idioma: En Revista: Scand J Immunol Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Suécia País de publicação: Reino Unido