[Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence].
Zhonghua Nan Ke Xue
; 11(4): 272-4, 277, 2005 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-15921257
OBJECTIVE: To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence. METHODS: Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining. RESULTS: At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells. CONCLUSION: Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complicações Pós-Operatórias
/
Prostatectomia
/
Incontinência Urinária
/
Transplante de Células
/
Músculo Esquelético
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
China