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Targeted disruption of iNOS prevents LPS-induced S-nitrosation of IRbeta/IRS-1 and Akt and insulin resistance in muscle of mice.
Carvalho-Filho, Marco A; Ueno, Mirian; Carvalheira, José B C; Velloso, Lício A; Saad, Mario J A.
Afiliação
  • Carvalho-Filho MA; Departamento de Clínica Médica, Universidade Estadual de Campinas, UNICAMP, 13081 970, Campinas SP, Brazil.
Am J Physiol Endocrinol Metab ; 291(3): E476-82, 2006 Sep.
Article em En | MEDLINE | ID: mdl-16638822
We have previously demonstrated that the insulin resistance associated with inducible nitric oxide synthase (iNOS) induction in two different models of obesity, diet-induced obesity and the ob/ob mice, is mediated by S-nitrosation of proteins involved in insulin signal transduction: insulin receptor beta-subunit (IRbeta), insulin receptor substrate 1(IRS-1), and Akt. S-nitrosation of IRbeta and Akt impairs their kinase activities, and S-nitrosation of IRS-1 reduces its tissue expression. In this study, we observed that LPS-induced insulin resistance in the muscle of wild-type mice, as demonstrated by reduced insulin-induced tyrosine phosphorylation of IRbeta and IRS-1, reduced IRS-1 expression and reduced insulin-induced serine phosphorylation of Akt. This resistance occurred in parallel with enhanced iNOS expression, which was accompanied by S-nitrosation of IRbeta/IRS-1 and Akt. In the muscle of iNOS(-/-) mice, we did not observe enhanced iNOS expression or any S-nitrosation of IRbeta/IRS-1 and Akt after LPS treatment. Moreover, insulin resistance was not present. The preservation of insulin-induced tyrosine phosphorylation of IRbeta and IRS-1, of IRS-1 protein expression, and of insulin-induced serine phosphorylation of Akt observed in LPS-treated iNOS(-/-) mice strongly suggests that the insulin resistance induced by LPS is iNOS mediated, probably through S-nitrosation of proteins of early steps of insulin signaling.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Resistência à Insulina / Receptor de Insulina / Lipopolissacarídeos / Óxido Nítrico Sintase Tipo II / Proteínas Proto-Oncogênicas c-akt Limite: Animals Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Resistência à Insulina / Receptor de Insulina / Lipopolissacarídeos / Óxido Nítrico Sintase Tipo II / Proteínas Proto-Oncogênicas c-akt Limite: Animals Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos