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Simultaneous determination of ginkgolides A, B, C and bilobalide in plasma by LC-MS/MS and its application to the pharmacokinetic study of Ginkgo biloba extract in rats.
Xie, Jiashu; Ding, Cungang; Ge, Qinghua; Zhou, Zhen; Zhi, Xiaojin.
Afiliação
  • Xie J; Shanghai Institute of Pharmaceutical Industry, National Pharmaceutical Engineering and Research Center, 1111 Halei Road, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci ; 864(1-2): 87-94, 2008 Mar 15.
Article em En | MEDLINE | ID: mdl-18295555
A new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of ginkgolides (includes ginkgolide C for the first time) and bilobalide in plasma is presented. Ketoprofen was used as an internal standard, and sample pre-treatment consisted of a liquid-liquid extraction. Chromatographic separation was achieved on a 5 microm Shiseido C8 column (150 mm x 2.0 mm i.d., particle size 5 microm) with a mobile phase consisting of methanol/ 6 mM ammonium acetate (60/40, v/v) at a flow rate of 0.3 ml/min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring (MRM) under negative ionization mode with an atmospheric pressure chemical ionization (APCI) interface. The method was validated in terms of intra- and inter-day precision (<12.7%), accuracy (within +/- 7.0%), linearity, specificity and stability. In addition, matrix effects of ginkgolides and bilobalide in plasma were evaluated in different reconstitution solvents. Smaller matrix effects were observed for reconstitution solvents containing less organic solvent. The method has been successfully applied to a pharmacokinetic study of Ginkgo biloba extract in rats after intravenous administration. This is the first report of pharmacokinetic data for ginkgolide C.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Cromatografia Líquida de Alta Pressão / Ginkgo biloba / Ciclopentanos / Ginkgolídeos / Espectrometria de Massas em Tandem / Furanos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Cromatografia Líquida de Alta Pressão / Ginkgo biloba / Ciclopentanos / Ginkgolídeos / Espectrometria de Massas em Tandem / Furanos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2008 Tipo de documento: Article País de publicação: Holanda