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Role of NtcA, a cyanobacterial global nitrogen regulator, in the regulation of sucrose metabolism gene expression in Anabaena sp. PCC 7120.
Marcozzi, Clarisa; Cumino, Andrea C; Salerno, Graciela L.
Afiliação
  • Marcozzi C; Centro de Investigaciones Biológicas and CEBB, Fundación para Investigaciones Biológicas Aplicadas, C.C. 1348, 7600 Mar del Plata, Argentina.
Arch Microbiol ; 191(3): 255-63, 2009 Mar.
Article em En | MEDLINE | ID: mdl-19082579
In the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 (also known as Nostoc sp. PCC 7120), it has been shown that spsB and susA, the genes coding for proteins related to sucrose synthesis and cleavage, respectively, exhibit converse expression regarding the nitrogen source. In the nitrogen-fixing filament, spsB expression is mostly localized to the heterocysts and susA is only expressed in vegetative cells. The aim of this work was to investigate the participation of NtcA, a global nitrogen regulator that operates in cyanobacteria, in the regulation of sucrose metabolism genes in Anabaena sp. PCC 7120. The induction of spsB expression observed in the filaments upon combined-nitrogen depletion was abolished in an NtcA-deficient mutant. In vitro experiments showed that NtcA binds specifically but with different affinities to two sites in the spsB promoter region. When susA expression was analyzed after a combined-nitrogen starvation, the levels of mRNA, polypeptide and activity increased in the mutant in comparison with the wild-type strain. Also, NtcA interacted with one site in the promoter region of susA. We conclude that sucrose metabolism is coordinated at the transcriptional level with nitrogen metabolism, suggesting a global metabolism regulating role for NtcA.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sacarose / Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Anabaena / Nitrogênio Idioma: En Revista: Arch Microbiol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sacarose / Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Anabaena / Nitrogênio Idioma: En Revista: Arch Microbiol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha