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Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens.
Hung, Hung-Chang; Chan, Chi-Ho; Tsao, Shih-Ming; Chen, Shiuan-Chih; Wu, Tzu-Chin; Lu, Min-Chi; Wu, Der-Jinn; Huang, Shu-Ju; Lee, Yuan-Ti.
Afiliação
  • Hung HC; Institute of Medicine, Chung Shan Medical University, No.110 Sec.1 Jianguo N. Rd.,Taichung, Taiwan.
Braz J Infect Dis ; 16(3): 242-9, 2012.
Article em En | MEDLINE | ID: mdl-22729191
OBJECTIVE: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. METHOD: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. RESULTS: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). The sensitivity and specificity of the DTB assay in detecting MTB in sputum samples was 77.2% and 100% compared to clinical diagnosis, while AFB smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. Compared to culture, DTB had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. Compared to clinical diagnosis, DTB had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 34.8% and 79.5%, respectively. CONCLUSIONS: The superior performance of the DTB assay relative to AFB smear microscopy makes it a valuable tool to enable early diagnosis of MTB, thereby improving patient care and reducing transmission.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Tuberculose Pulmonar / Líquido da Lavagem Broncoalveolar / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Guideline / Screening_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Middle aged Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Tuberculose Pulmonar / Líquido da Lavagem Broncoalveolar / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Guideline / Screening_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Female / Humans / Infant / Middle aged Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Brasil