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Comparison of frozen and unfrozen blood spots for gene expression studies.
Wei, Changshuai; Lu, Qing; Khoo, Sok Kean; Lenski, Madeleine; Fichorova, Raina N; Leviton, Alan; Paneth, Nigel.
Afiliação
  • Wei C; Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
  • Lu Q; Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
  • Khoo SK; Laboratory of Microarray Technology, Van Andel Research Institute, Grand Rapids, MI.
  • Lenski M; Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
  • Fichorova RN; Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School and Brigham and Women's Hospital, Boston, MA.
  • Leviton A; Neuroepidemiology Unit, Department of Neurology, Boston Children's Hospital, Harvard University, Boston, MA.
  • Paneth N; Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI; Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI. Electronic address: paneth@msu.edu.
J Pediatr ; 164(1): 189-191.e1, 2014 Jan.
Article em En | MEDLINE | ID: mdl-24209717
We studied gene expression in 9 sets of paired newborn blood spots stored for 8-10 years in either the frozen state or the unfrozen state. Fewer genes were expressed in unfrozen spots, but the average correlation coefficient for overall gene expression comparing the frozen and unfrozen state was 0.771 (95% CI, 0.700-0.828).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Criopreservação / Triagem Neonatal / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Congelamento Limite: Humans / Newborn Idioma: En Revista: J Pediatr Ano de publicação: 2014 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Criopreservação / Triagem Neonatal / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Congelamento Limite: Humans / Newborn Idioma: En Revista: J Pediatr Ano de publicação: 2014 Tipo de documento: Article País de publicação: Estados Unidos