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The ratio of transforming growth factor-ß1/bone morphogenetic protein-7 in the progression of the epithelial-mesenchymal transition contributes to rat liver fibrosis.
Bi, W R; Xu, G T; Lv, L X; Yang, C Q.
Afiliação
  • Bi WR; Department of Gastroenterology and Digestive Disease Institute, Tongji Hospital Branch, Tongji University School of Medicine, Shanghai, China.
  • Xu GT; Department of Stem Cells Laboratory, Tongji University School of Medicine, Shanghai, China.
  • Lv LX; Department of Stem Cells Laboratory, Tongji University School of Medicine, Shanghai, China.
  • Yang CQ; Department of Gastroenterology and Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China cqyang@tongji.edu.cn.
Genet Mol Res ; 13(1): 1005-14, 2014 Feb 20.
Article em En | MEDLINE | ID: mdl-24634122
This study was designed to show whether rat liver epithelial cells could undergo epithelial-mesenchymal transition (EMT), thereby directly contributing to liver fibrosis. The role of the ratio of transforming growth factor-ß1 (TGF-ß1)/bone morphogenetic protein-7 (BMP-7) was evaluated in the progression of EMT or mesenchymal-epithelial transition. Primary rat liver epithelial cells were stimulated with different ratios of TGF-ß1/BMP-7 and examined for evidence of transition to a mesenchymal or epithelial phenotype. Liver sections were labeled to detect antigens associated with liver epithelial cells [E-cadherin (E-cad)], EMT [fibroblast-specific protein-1 (FSP-1), vimentin], myofibroblasts [α-smooth muscle actin (α-SMA)], and intracellular signal-transduction mediated by forming liver fibrosis undergo EMT, resulting in the formation of invasive fibroblasts; this process may be driven or impeded by a response to local TGF-ß1 or BMP-7. BMP-7 downregulated α-SMA and phosphorylated Smad2/3. Stimulation of cultured cells with TGF-ß1 induced the expression of pSmad2/3, FSP-1, and α-SMA. Stimulation of cultured cells with BMP-7 induced the expression of E-cad. We demonstrated that the cells upregulated E-cad release compared with untreated cells, but TGF-ß1 was different. We found that the equilibrium of the ratio of TGF-ß1/BMP-7 was 1/10. In summary, the mechanism for this process was not determined. Demonstration of the contribution of what the ratio of TGF-ß1/BMP-7 induced to EMT to the chronic liver diseases would provide a new basis for understanding pathogenesis and potential treatment.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator de Crescimento Transformador beta1 / Proteína Morfogenética Óssea 7 / Transição Epitelial-Mesenquimal / Fígado Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator de Crescimento Transformador beta1 / Proteína Morfogenética Óssea 7 / Transição Epitelial-Mesenquimal / Fígado Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil