Your browser doesn't support javascript.
loading
Expression of serum exosomal microRNA-21 in human hepatocellular carcinoma.
Wang, Hongwei; Hou, Lijuan; Li, Aihui; Duan, Yuxiu; Gao, Haili; Song, Xinwen.
Afiliação
  • Wang H; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
  • Hou L; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
  • Li A; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
  • Duan Y; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
  • Gao H; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
  • Song X; Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, China.
Biomed Res Int ; 2014: 864894, 2014.
Article em En | MEDLINE | ID: mdl-24963487
New strategies for the diagnosis of hepatocellular carcinoma (HCC) are urgently needed. There is an increasing interest in using microRNAs (miRNAs) as biomarkers in diseases. In this study, we examined the expression of miR-21 in serum exosomes from patients with HCC or chronic hepatitis B (CHB) and investigated the potential clinical significance of miR-21. Quantitative RT-PCR indicated that the concentration of miR-21 was significantly higher in exosomes than in exosome-depleted supernatants or the whole serum. Further, the expression level of serum exosomal miR-21 was significantly higher in patients with HCC than those with CHB or healthy volunteers (P < 0.0001, P < 0.0001, resp.). High level of miR-21 expression correlated with cirrhosis (P = 0.024) and advanced tumor stage (P = 0.001). Although serum level of miR-21 was higher in patients with HCC than in patients with CHB and healthy volunteers, the sensitivity of detection is much lower than using exosomal miR-21. These findings indicate that miR-21 is enriched in serum exosomes which provides increased sensitivity of detection than whole serum. Exosomal miR-21 may serve as a potential biomarker for HCC diagnosis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Neoplásico / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Carcinoma Hepatocelular / MicroRNAs / Exossomos / Neoplasias Hepáticas Tipo de estudo: Diagnostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Res Int Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Neoplásico / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / Carcinoma Hepatocelular / MicroRNAs / Exossomos / Neoplasias Hepáticas Tipo de estudo: Diagnostic_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Res Int Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos