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Comparison of extraction methods of total microbial DNA from freshwater.
Li, P; Yang, S F; Lv, B B; Zhao, K; Lin, M F; Zhou, S; Song, X; Tang, X M.
Afiliação
  • Li P; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Yang SF; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Lv BB; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Zhao K; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Lin MF; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Zhou S; Institute of ECO-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Song X; Institute of ECO-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.
  • Tang XM; Biotech Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, China sunsite@126.com.
Genet Mol Res ; 14(1): 730-8, 2015 Jan 30.
Article em En | MEDLINE | ID: mdl-25730010
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water. DNA extraction was analyzed by agarose gel electrophoresis, ultraviolet-spectroscopy, restriction enzyme digestion, and polymerase chain reaction. The results showed that the modified filtration-membrane method was the most efficient for extracting microbial DNA from freshwater with high integrity and purity and is suitable for molecular applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / DNA Bacteriano / Centrifugação / Cromatografia em Gel Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / DNA Bacteriano / Centrifugação / Cromatografia em Gel Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil