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Insulin complexation with hydroxypropyl-beta-cyclodextrin: Spectroscopic evaluation of molecular inclusion and use of the complex in gel for healing of pressure ulcers.
Valentini, Sóstenes Rosa; Nogueira, Ana Cláudia; Fenelon, Vanderson Carvalho; Sato, Francielle; Medina, Antonio N; Santana, Rosângela Getirana; Baesso, Mauro Luciano; Matioli, Graciette.
Afiliação
  • Valentini SR; Pharmacy Department, State University of Maringá, Paraná, Av. Colombo 5790, Maringá, Paraná 87020-900, Brazil.
  • Nogueira AC; Physics Department, State University of Maringá, Paraná, Brazil.
  • Fenelon VC; Pharmacy Department, State University of Maringá, Paraná, Av. Colombo 5790, Maringá, Paraná 87020-900, Brazil.
  • Sato F; Physics Department, State University of Maringá, Paraná, Brazil.
  • Medina AN; Physics Department, State University of Maringá, Paraná, Brazil.
  • Santana RG; Statistics Department, State University of Maringá, Paraná, Brazil.
  • Baesso ML; Physics Department, State University of Maringá, Paraná, Brazil.
  • Matioli G; Pharmacy Department, State University of Maringá, Paraná, Av. Colombo 5790, Maringá, Paraná 87020-900, Brazil. Electronic address: gmatioli@uem.br.
Int J Pharm ; 490(1-2): 229-39, 2015 Jul 25.
Article em En | MEDLINE | ID: mdl-25987212
The pressure ulcer healing is a complex process and difficult to be achieved. Insulin is known to promote wound healing, and when complexed with cyclodextrin presents improved solubility, stability and biological activity. Complexation of insulin with hydroxypropyl-beta-cyclodextrin (HPßCD) was performed in this work through the coprecipitation method, providing the inclusion complex (HPßCD-I). The spectroscopic techniques used to analyze the complex were H(1) NMR, FT-Raman and FT-IR/ATR. A gel containing the HPßCD-I complex was prepared and a clinical study was conducted in patients with pressure ulcers. The spectroscopic techniques allowed to confirm the complex formation through the inclusion of aromatic amino acids, such as phenylalanine present in the HPßCD cavity. Data obtained from the FT-Raman and FT-IR/ATR techniques, combined with the H(1) NMR results, showed the effectiveness of these techniques in evaluating the inclusion complex of HPßCD with insulin. Clinical studies demonstrated tissue revitalization and a trend (p=0.06) for a significant difference between the healing effect of the control gel and that with HPßCD-I complex. The creation of the gel prepared with insulin and HPßCD-I complex and its use in patients with pressure ulcers appears to be promising in wound healing and its possible use in hospital care.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Úlcera por Pressão / Beta-Ciclodextrinas / Insulina Limite: Aged / Humans / Middle aged Idioma: En Revista: Int J Pharm Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Úlcera por Pressão / Beta-Ciclodextrinas / Insulina Limite: Aged / Humans / Middle aged Idioma: En Revista: Int J Pharm Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda