Self-amplified BDNF transcription is a regulatory system for synaptic maturation in cultured cortical neurons.
Neurochem Int
; 91: 55-61, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26596846
Neuronal cell survival and synaptic plasticity are controlled through expression of various neurotrophic factors including brain-derived neurotrophic factor (BDNF). In the present study, we examined the mechanism behind BDNF-induced Bdnf mRNA production and the physiological role of its amplification system using cortical neurons. Exogenous BDNF was applied to the cultured cortical neurons at days in vitro (DIV) 3 and DIV 7 with or without inhibitors for intracellular signaling. Expression levels of total Bdnf and Bdnf variants (exon I, exon IV, and exon VI) were biphasically increased after the BDNF application in different developing stage of neurons. Inhibitor for extracellular signal-regulated kinase, calmodulin dependent protein kinase II, or protein kinase A repressed the BDNF-induced Bdnf mRNA expression. Furthermore, we found that application of TrkB-Fc, which scavenges produced endogenous BDNF, resulted in weakened BDNF/TrkB signaling and decreased expression of postsynaptic proteins, suggesting that newly synthesized BDNF induced by the self-amplification system contributes to the synaptic maturation and function.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Córtex Cerebral
/
Fator Neurotrófico Derivado do Encéfalo
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Neurochem Int
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
Reino Unido