Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency.

Dang, Ying; Jia, Gengxiang; Choi, Jennie; Ma, Hongming; Anaya, Edgar; Ye, Chunting; Shankar, Premlata; Wu, Haoquan

Dang, Ying; Jia, Gengxiang; Choi, Jennie; Ma, Hongming; Anaya, Edgar; Ye, Chunting; Shankar, Premlata; Wu, Haoquan.

Afiliação

Dang Y; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. ying.dang@ttuhsc.edu.
Jia G; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. gengxiang.jia@ttuhsc.edu.
Choi J; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. jenniechoi@gmail.com.
Ma H; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. hongming.ma@ttuhsc.edu.
Anaya E; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. edgar.anaya@ttuhsc.edu.
Ye C; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. chunting.ye@ttuhsc.edu.
Shankar P; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. premlata.shankar@ttuhsc.edu.
Wu H; Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA. haoquan.wu@ttuhsc.edu.

Genome Biol ; 16: 280, 2015 Dec 15.

Article em En | MEDLINE | ID: mdl-26671237

Assuntos

Sistemas CRISPR-Cas; Técnicas de Inativação de Genes; RNA/química; Proteínas Associadas a CRISPR/genética; Linhagem Celular; Desoxirribonuclease I/genética; Deleção de Genes; Humanos; Células Jurkat; Mutação

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA / Técnicas de Inativação de Genes / Sistemas CRISPR-Cas Limite: Humans Idioma: En Revista: Genome Biol Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

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