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The cyclic-di-GMP diguanylate cyclase CdgA has a role in biofilm formation and exopolysaccharide production in Azospirillum brasilense.
Ramírez-Mata, Alberto; López-Lara, Lilia I; Xiqui-Vázquez, Ma Luisa; Jijón-Moreno, Saúl; Romero-Osorio, Angelica; Baca, Beatriz E.
Afiliação
  • Ramírez-Mata A; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: aramirezmata@gmail.com.
  • López-Lara LI; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: 13_isela13@hotmail.com.
  • Xiqui-Vázquez ML; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: luisa.xiqui@hotmail.com.
  • Jijón-Moreno S; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: jims_pcm@hotmmail.com.
  • Romero-Osorio A; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: angy.4behappy@gmail.com.
  • Baca BE; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103J, Av. San Claudio S/N, Col. San Manuel, Puebla Pue CP 72570, Mexico. Electronic address: beatriz.baca@correo.buap.mx.
Res Microbiol ; 167(3): 190-201, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26708984
In bacteria, proteins containing GGDEF domains are involved in production of the second messenger c-di-GMP. Here we report that the cdgA gene encoding diguanylate cyclase A (CdgA) is involved in biofilm formation and exopolysaccharide (EPS) production in Azospirillum brasilense Sp7. Biofilm quantification using crystal violet staining revealed that inactivation of cdgA decreased biofilm formation. In addition, confocal laser scanning microscopy analysis of green-fluorescent protein-labeled bacteria showed that, during static growth, the biofilms had differential levels of development: bacteria harboring a cdgA mutation exhibited biofilms with considerably reduced thickness compared with those of the wild-type Sp7 strain. Moreover, DNA-specific staining and treatment with DNase I, and epifluorescence studies demonstrated that extracellular DNA and EPS are components of the biofilm matrix in Azospirillum. After expression and purification of the CdgA protein, diguanylate cyclase activity was detected. The enzymatic activity of CdgA-producing cyclic c-di-GMP was determined using GTP as a substrate and flavin adenine dinucleotide (FAD(+)) and Mg(2)(+) as cofactors. Together, our results revealed that A. brasilense possesses a functional c-di-GMP biosynthesis pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Azospirillum brasilense / GMP Cíclico / Biofilmes / Fósforo-Oxigênio Liases / Proteínas de Escherichia coli País/Região como assunto: America do sul / Brasil Idioma: En Revista: Res Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de publicação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Azospirillum brasilense / GMP Cíclico / Biofilmes / Fósforo-Oxigênio Liases / Proteínas de Escherichia coli País/Região como assunto: America do sul / Brasil Idioma: En Revista: Res Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de publicação: França